49 research outputs found

    Improving Community Capacity in Rapid Disaster Mapping: An Evaluation of Summer School

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    Experiences with natural disasters have intensified recent efforts to enhance cooperation mechanisms among official disaster management institutions to community participation. These experiences reveal a need to enhance rapid mapping technical assistance to be developed and shared among young scientists through a summer school. However, the question arose of how effective this summer school to be used as a tool to increase scientists’ understanding and capacity. This study sought to evaluate the extent to which human resource capacity building can be effectively implemented. The methods used for this evaluation is through observations, questionnaires and a weighted scoring based on knowledge, skills and attitudes’ criteria. The results indicate a significant improvement in knowledge (94.56%), skills (82%) and attitudes (85.20%) among the participants. Even though there are still gaps in participants’ skills, the summer school was found to be an effective way to train the young scientists for rapid mapping

    Hydrophobically Modified Sulfobetaine Copolymers with Tunable Aqueous UCST through Postpolymerization Modification of Poly(pentafluorophenyl acrylate)

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    Polysulfobetaines, polymers carrying highly polar zwitterionic side chains, present a promising research field by virtue of their antifouling properties, hemocompatibility, and stimulus-responsive behavior. However, limited synthetic approaches exist to produce sulfobetaine copolymers comprising hydrophobic components. Postpolymerization modification of an activated ester precursor, poly(pentafluorophenyl acrylate), employing a zwitterionic amine, 3-((3-aminopropyl)dimethylammonio)propane-1-sulfonate, ADPS, is presented as a novel, one-step synthetic concept toward sulfobetaine (co)polymers. Modifications were performed in homogeneous solution using propylene carbonate as solvent with mixtures of ADPS and pentylamine, benzylamine, and dodecylamine producing a series of well-defined statistical acrylamido sulfobetaine copolymers containing hydrophobic pentyl, benzyl, or dodecylacrylamide comonomers with well-controllable molar composition as evidenced by NMR and FT-IR spectroscopy and size exclusion chromatography.This synthetic strategy was exploited to investigate, for the first time, the influence of hydrophobic modification on the upper critical solution temperature (UCST) of sulfobetaine copolymers in aqueous solution. Surprisingly, incorporation of pentyl groups was found to increase solubility over a wide composition range, whereas benzyl groups decreased solubility—an effect attributed to different entropic and enthalpic contributions of both functional groups. While UCST transitions of polysulfobetaines are typically limited to higher molar mass samples, incorporation of 0–65 mol % of benzyl groups into copolymers with molar masses of 25.5–34.5 kg/mol enabled sharp, reversible transitions from 6 to 82 °C in solutions containing up to 76 mM NaCl, as observed by optical transmittance and dynamic light scattering. Both synthesis and systematic UCST increase of sulfobetaine copolymers presented here are expected to expand the scope and applicability of these smart materials

    Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

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    In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

    AN INTRODUCTION OF SURVEYING AND GEOMATICS EDUCATION WITH E-PLATFORM IN NCTU, TAIWAN

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    This article presents the current status of Surveying and Geomatics education provided by Department of Civil Engineering at National Chiao Tung University, Taiwan. The Surveying and Geomatics Education at NCTU is introduced first. Then the current status of using E-learning platform for Surveying and Geomatics courses is described. This platform, also known as E-Campus, is designed and implemented by NCTU Digital Content Production Center. This paper also shows some statistical numbers of Surveying and Geomatics courses using E-Campus. The practical results indicated that the average login for undergraduate student is from 38 to 60 times per student for each course while the average login for graduate student is from 69 to 105

    A Study on Factors Affecting Airborne LiDAR Penetration

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    This study uses data from different periods, areas and parameters of airborne LiDAR (light detection and ranging) surveys to understand the factors that influence airborne LiDAR penetration rate. A discussion is presented on the relationships between these factors and LiDAR penetration rate. The results show that the flight height above ground level (AGL) does not have any relationship with the penetration rate. There are some factors that should have larger influence. For example, the laser is affected by a wet ground surface by reducing the number of return echoes. The field of view (FOV) has a slightly negative correlation with the penetration rate, which indicates that the laser incidence angle close to zero should achieve the best penetration. The vegetation cover rate also shows a negative correlation with the penetration rate, thus bare ground and reduced vegetation in the aftermath of a typhoon also cause high penetration rate. More return echoes could be extracted from the full-waveform system, thereby effectively improving the penetration rate. This study shows that full-waveform LiDAR is an effective method for increasing the number of surface reflected echoes. This study suggests avoiding LiDAR survey employment directly following precipitation to prevent laser echo reduction

    Spatial-Planning-Based Ecosystem Adaptation (SPBEA): A Concept and Modeling of Prone Shoreline Retreat Areas

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    Ecosystem-based adaptation to climate change impacts, such as shoreline retreat, has been promoted at the international, national, and even local levels. However, among scientists, opinions about how to implement it in spatial-planning practices are varied. Science-based environmental factors, human wellbeing, and sustainable development can be strengthened by developing spatial-planning-based ecosystem adaptations (SPBEAs). Therefore, this article aims to assess how the SPBEA model can be developed within an area prone to shoreline retreat. A coastal area of the Sayung subdistrict in Central Java, Indonesia, was selected as a study area because it has experienced a massive shoreline retreat. A multicriteria analysis (MCA) method was employed for developing the model by using the geographic information system (GIS) technique of analysis, divided into three steps: the fishpond zone determination, which involved the analytical hierarchy process (AHP) method in the process of model development; the fishpond site determination; SPBEA fishpond site development. The results show that the SPBEA model is the best practice solution for combatting shoreline retreat because of tidal waves and/or sea-level rise. The spatial site management should empower the coastal protection zone and the sustainable fishpond zone by implementing a silvofishery approach
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